Effects of Pulsed Light on Mycelium Growth and Conidiation in Aspergillus oryzae
雜志:Fermentation-basel
出版年月:2023-7
摘要(英譯中):了解米曲霉的光響應(yīng)機(jī)制對(duì)釀造和余料處理行業(yè)制定有效的光調(diào)控方案至關(guān)重要。雖然已知連續(xù)光照會(huì)限制米曲霉的生長,但米曲霉對(duì)含光照期的光照的敏感性知之甚少。在這項(xiàng)研究中,我們使用脈沖波調(diào)制(PWM)技術(shù)產(chǎn)生了9種脈沖藍(lán)光(PBL)處理,這些脈沖藍(lán)光具有不同的峰值光強(qiáng)度和頻率。然后我們比較了連續(xù)藍(lán)光(CBL)對(duì)米曲霉的影響。研究結(jié)果表明,GDMCC 3.31米曲霉在光劑量和平均光強(qiáng)恒定不變的情況下,其菌絲在特定峰值強(qiáng)度和頻率的PBL下生長速度快,且分生孢子產(chǎn)量多。其中,兩種PBL處理(PBL-20_40%_1 Hz和PBL-400_20%_10 kHz)下的菌落直徑和分生孢子數(shù)分別是CBL下米曲霉的1.13倍和1.22倍。這種不同的反應(yīng)可能主要?dú)w因于米曲霉對(duì)自然界光暗循環(huán)的適應(yīng)。此外,還發(fā)現(xiàn)峰值光強(qiáng)和頻率之間存在交互作用。本研究將脈沖波調(diào)制作為影響米曲霉光響應(yīng)的新因素,并將其應(yīng)用于發(fā)酵光調(diào)節(jié)方法的開發(fā)中。
摘要圖:
Figure 3. Colony diameters upon PBL and CBL treatments. Subfigures (a), (b), and (c) are the results of the mycelium growth under PBL with different peak intensities if the frequency was held constant. Subfigures (d), (e), and (f) are the results of the mycelium growth under PBL with different frequencies if the peak intensity was kept unchanged. The symbols *, **, ***, and **** in the figure represent P<0.05, P<0.01, P<0.001, and P<0.0001, respectively. The label PL-100_80%_1 Hz represents a PBL with a peak intensity of 100 μmol×m-2s-1, a duty ratio of 80%, and a frequency of 1 Hz. Other labels are shown in a similar way.